Biosynthesis of glycolipid from glucosamine and C_(13:0) fatty acid was attempted using an enzyme preparation which was extracted from Selenomonas ruminantium grown in lactic acid medium by means of ultrasonication, and with cofactors of ATP, Co A, Mg^(++), and UTP. The results are summarized as follows:
1. The rate of synthesis of glycolipid from ^(14)C-glucosamine and tridecyl CoA by an enzyme from Selenomonas ruminantium was promoted by the presence of co-factors, ATP, Mg, and UTP.
2. The rate of incorporation of ^(14)C-glucosamine into glycolipid by the centrifugal fraction of bacterial preparation was the highest in the 105,000 g supernatant fraction, indicating about twice the enzyme activity as the 6, 000 rpm supernatant fraction.
3. The biosynthesis of glycolipid from ^(14)C-glucosamine and tridecyl-CoA by the crude enzyme of the 105,000 g supernatant fraction of Selenomonas ruminantium cell preparation proceeded the most part of it in 30 minutes and completed in an hour.
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